Journal: Microbial Biotechnology

Article Title: Mutualistic interaction between Salmonella enterica and Aspergillus niger and its effects on Zea mays colonization

doi: 10.1111/1751-7915.12182

Figure Lengend Snippet: Aspergillus niger remains alive when colonized by S . Typhimurium. Epifluorescence microscopy overlay images of live and heat-killed A. niger mycelia (filaments, grey) co-incubated with sfGFP-labelled S . Typhimurium cells (false coloured green, left panels) stained with propidium iodide (white, right panels). Scale bars: 50 μm.

Article Snippet: Strain RB164 harbours a constitutively expressed superfolder green fluorescent protein (sfGFP) (Pédelacq et al ., ) inserted in the chromosomal pseudogene locus malX-malY (Jarvik et al ., ).

Techniques: Epifluorescence Microscopy, Incubation, Staining

Journal: Microbial Biotechnology

Article Title: Mutualistic interaction between Salmonella enterica and Aspergillus niger and its effects on Zea mays colonization

doi: 10.1111/1751-7915.12182

Figure Lengend Snippet: Bacteria protect A. niger from killing by cycloheximide. Epifluorescence microscopy overlay images of A. niger mycelia (filaments, grey) grown alone or co-incubated with wild-type or ΔcsgD mutant S . Typhimurium cells tagged with sfGFP (false coloured green) for 48 h. Samples were either untreated or exposed to cycloheximide for 12 h prior to staining with propidium iodide (white). Scale bars: 50 μm.

Article Snippet: Strain RB164 harbours a constitutively expressed superfolder green fluorescent protein (sfGFP) (Pédelacq et al ., ) inserted in the chromosomal pseudogene locus malX-malY (Jarvik et al ., ).

Techniques: Bacteria, Epifluorescence Microscopy, Incubation, Mutagenesis, Staining

Journal: Microbial Biotechnology

Article Title: Mutualistic interaction between Salmonella enterica and Aspergillus niger and its effects on Zea mays colonization

doi: 10.1111/1751-7915.12182

Figure Lengend Snippet: Bacteria require live fungus to form a biofilm. In vitro co-incubation of live and heat-killed mycelia of A. niger with sfGFP-labelled S . Typhimurium cells (false coloured green). Images are overlay of transmitted light (grey) with GFP fluorescence.A. Time-course of biofilm initiation on fungal hyphae (blue arrows) over a period of 24 h.B. Dense biofilms are formed on live hyphae (upper panels) after 6 h and are maintained for 15 days. In contrast, bacterial attachment to heat-killed mycelia (lower panels) is limited and disappears with time. Scale bars: 50 μm.

Article Snippet: Strain RB164 harbours a constitutively expressed superfolder green fluorescent protein (sfGFP) (Pédelacq et al ., ) inserted in the chromosomal pseudogene locus malX-malY (Jarvik et al ., ).

Techniques: Bacteria, In Vitro, Incubation, Fluorescence

Journal: Microbial Biotechnology

Article Title: Mutualistic interaction between Salmonella enterica and Aspergillus niger and its effects on Zea mays colonization

doi: 10.1111/1751-7915.12182

Figure Lengend Snippet: Maize roots are colonized by S . Typhimurium and A. niger . Images are overlay of transmitted light (grey) with sfGFP fluorescence (false coloured green).A. Epidermal maize root tissue colonized by sfGFP-labelled S . Typhimurium.B. Epidermal maize root tissue colonized by A. niger (blue arrow).C. Epidermal maize root tissue colonized by sfGFP-labelled S . Typhimurium and A. niger . Blue arrow points at a representative fungal filament.D. Non-inoculated maize root tissue is shown as control. Scale bars: 50 μm.

Article Snippet: Strain RB164 harbours a constitutively expressed superfolder green fluorescent protein (sfGFP) (Pédelacq et al ., ) inserted in the chromosomal pseudogene locus malX-malY (Jarvik et al ., ).

Techniques: Fluorescence, Control